Enzyme Assay in Microsomes Below Zero Degrees | Zendy

Pascale Debey | Zendy; Claude Balny | Zendy; Pierre Douzou | Zendy

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Enzyme Assay in Microsomes Below Zero Degrees

Author(s) -

Pascale Debey,

Claude Balny,

Pierre Douzou

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.9.2633

Subject(s) - ethylene glycol , microsome , aqueous solution , antifreeze , chemistry , enzyme , solvent , chromatography , electron transfer , electron transport chain , membrane , biochemistry , organic chemistry

Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .

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