Open AccessSome Binding Parameters of Chromatin Acidic Proteins with High Affinity for Deoxyribonucleic Acid
Author(s) -
Gordhan L. Patel,
Terry L. Thomas
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.9.2524
Subject(s) - dna , chromatin , ionic strength , biochemistry , heterologous , biology , dna binding protein , fraction (chemistry) , in vitro , homologous chromosome , microbiology and biotechnology , chemistry , chromatography , gene , aqueous solution , transcription factor
A fraction of chromatin acidic protein from rat liver, predominantly comprised of low-molecular-weight species, showed highestin vitro affinity for DNA when compared with other fractions of nuclear acidic protein. Maximum binding of this protein fraction was observed at physiological ionic strength of 0.14 M NaCl and DNA/protein ratio > 1. Comparison of their binding to homologous and heterologous DNAs showed partial species specificity. All molecular species of this protein fraction appear to bind to DNA.