Open AccessDetection and Isolation of a New DNA Polymerase from Human Breast Tumor Cell Line HBT-3 by (dT) 12-18 -Cellulose Chromatography
Author(s) -
Brenda I. Gerwin,
Robert H. Bassin
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.8.2453
Subject(s) - polymerase , cellulose , dna , cell culture , dna polymerase , microbiology and biotechnology , enzyme , human breast , column chromatography , cell , biology , chemistry , chromatography , biochemistry , genetics , breast cancer , cancer
Column chromatography on (dT)12-18 -cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A)·(dT)12-18 as compared to either poly(dA)·(dT)12-18 or poly(A)·poly(dT), and ability to copy poly(C)·(dG)12-18 . A similar DNA polymerase activity, defined by its binding to (dT)12-18 -cellulose, is not detectable in several other cell lines of human tumors.