Open AccessGrowth- and Density-Dependent Inhibition of Deoxyglucose Transport in Balb 3T3 Cells and Its Absence in Cells Transformed by Murine Sarcoma Virus
Author(s) -
S. K. Bose,
Barbara J. Zlotnick
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.8.2374
Subject(s) - deoxyglucose , 3t3 cells , cell culture , theophylline , biology , growth inhibition , contact inhibition , cell growth , virus , microbiology and biotechnology , endocrinology , virology , biochemistry , transfection , genetics
The earliest measurable parameter that shows density-dependent inhibition is the uptake of [3 H]deoxyglucose by Balb 3T3 cells. The rate decreases even during the exponential phase of growth and reaches a minimum, about 8- to 10-times lower than maximum, as the culture approaches the saturation density. Cells transformed by murine sarcoma virus fail to show either growth-dependent or density-dependent inhibition of deoxyglucose uptake. Treatment with dibutyryl cyclic AMP in the presence of theophylline results in premature cessation of growth and in an arrest in the decline of deoxyglucose transport. Culture in serum-deficient medium also produces rapid inhibition of growth at low cell density, but these cultures exhibit a markedly decreased rate of deoxyglucose uptake.