Open AccessStudies on In Vitro DNA Synthesis Purification of dna C Gene Product Containing dna D Activity from Escherichia coli
Author(s) -
Sue Wickner,
Ira Berkower,
Michel Wright,
Jerard Hurwitz
Publication year - 1973
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.8.2369
Subject(s) - dna , dna polymerase ii , microbiology and biotechnology , biology , escherichia coli , dna clamp , dna replication , in vitro recombination , gene , circular bacterial chromosome , mutant , complementation , gene product , biochemistry , molecular cloning , gene expression , polymerase chain reaction , reverse transcriptase
The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The dna C and dna D gene products copurify, have similar characteristics, are both temperature sensitive in preparations from dna C temperature-sensitive cells, and are both undetectable in preparations from dna D temperature-sensitive cells.
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