RNA Polymerase from Bacillus amyloliquefaciens Infected with ϕ29 Bacteriophage | Zendy

Michael J. Holland | Zendy; H.R. Whiteley | Zendy

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RNA Polymerase from Bacillus amyloliquefaciens Infected with ϕ29 Bacteriophage

Author(s) -

Michael J. Holland,

H.R. Whiteley

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.8.2234

Subject(s) - polymerase , bacteriophage , bacillus amyloliquefaciens , rna polymerase , biology , microbiology and biotechnology , dna polymerase , rna dependent rna polymerase , enzyme , dna , rna , virology , bacteria , gene , biochemistry , genetics , escherichia coli

DNA-dependent RNA polymerase was purified from uninfected and ϕ29-infectedBacillus amyloliquefaciens . Differences were observed in the specific activities, template specificities, stability, and sedimentation properties of the two enzymes. A polypeptide of 30,000 molecular weight was found in association with the polymerase of high specific activity from phage-infected cells and was absent from polymerase isolated from uninfected cells. The change in polymerase properties and the appearance of the polypeptide occurred early in phage infection.

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