Open AccessControl of Chondrogenesis in Limb-Bud Cell Cultures by Bromodeoxyuridine
Author(s) -
Daniel Levitt,
Albert Doreman
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.8.2201
Subject(s) - glycosaminoglycan , chondroitin sulfate , chondrogenesis , limb bud , bromodeoxyuridine , dna synthesis , biochemistry , mesenchyme , cartilage , chemistry , keratan sulfate , heparan sulfate , biology , microbiology and biotechnology , cell growth , cell , dna , anatomy , embryo , gene
Initial exposure of cultured limb-bud cells (stage 23-24) to 5-bromo-2′-deoxyuridine (BrdU) irreversibly inhibits differentiation to cartilage under three different culture conditions. The inhibition of chondroitin sulfate synthesis is partially reversed by D-xylose in limb-bud cells after treatment with BrdU. The activities of four enzymes involved in chondroitin sulfate production were reduced in BrdU-treated cultures, but the magnitude of decrease was far less than the decrease in glycosaminoglycan synthesis. The slight increase in the turnover rate of sulfated glycosaminoglycans in BrdU-treated mesenchyme was not sufficient to account for the marked decrease in chondroitin sulfate content. The results suggest that BrdU treatment interferes with normal synthesis of chondroitin sulfate core protein in cultured limb-bud cells, but does not greatly diminish enzyme activities or UDP-sugar levels necessary for production of polysaccharide chains.