Effects of Selective Extraction of Histones on Template Activities of Chromatin by Use of Exogenous DNA and RNA Polymerases | Zendy

A. E. Mirsky | Zendy; B. D. Silverman | Zendy

Open Access

Effects of Selective Extraction of Histones on Template Activities of Chromatin by Use of Exogenous DNA and RNA Polymerases

Author(s) -

A. E. Mirsky,

B. D. Silverman

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.7.1973

Subject(s) - histone , chromatin , polymerase , dna , biology , microbiology and biotechnology , dna polymerase , rna , rna polymerase , histone h2a , histone modifying enzymes , biochemistry , chemistry , gene

Exogenous DNA and RNA polymerases were used to measure the template activity of DNA in chromatin in isolated thymus nuclei from which lysinerich or arginine-rich histones were selectively extracted. Measurements were on nuclei containing 20-1200 mug of DNA per ml, the distinctions becoming clear at the higher concentrations. Experiments with RNA polymerase showed only moderate increases in template activity upon extraction of histone, although the removal of lysinerich histone had a greater effect than that of arginine-rich histone. DNA polymerase action on nuclei minus lysinerich histone achieved high results, exceeding even those on DNA itself.

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