Open AccessEffect of Nalidixic Acid on DNA Replication by Toluene-Treated Escherichia coli
Author(s) -
Richard M. Burger,
Donald A. Glaser
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.7.1955
Subject(s) - nalidixic acid , semiconservative replication , in vivo , escherichia coli , dna replication , dna , toluene , chemistry , dna synthesis , biology , biochemistry , microbiology and biotechnology , genetics , origin of replication , organic chemistry , gene
Nalidixic acid inhibits DNA synthesis in toluene-treatedE. coli , strain B/r, as it is well known to doin vivo . Both semiconservative and repair syntheses are affected, though to different degrees. Density-transfer experiments indicate that chromosomal replication is reinitiated when nalidixic acid is removed from toluene-treated cells after exposure to the acid for one generationin vivo . For cellsin vivo or after toluene-treatment, reinitiation is not seen in asynchronous cultures exposed briefly to nalidixic acid or in cells prevented from synthesizing proteins during their exposure to the acid. Reinitiation occurs at the chromosomal origin but, unlike the effect seenin vivo , replication at the old site persists.