Open AccessPurification of Oncornaviruses by Agglutination with Concanavalin A
Author(s) -
Margaret L. Stewart,
Donald F. Summers,
Ruy Soeiro,
Bernard N. Fields,
Jacob V. Maizel
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.5.1308
Subject(s) - infectivity , concanavalin a , virus , agglutination (biology) , centrifugation , nucleic acid , chemistry , malachite green , glycoprotein , virology , biology , microbiology and biotechnology , biochemistry , antibody , immunology , in vitro , organic chemistry , adsorption
Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with α-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm3 were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm3 , indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness.