Open AccessThe 3′-Terminal Nucleotide Sequences of Bacteriophage λ DNA
Author(s) -
Paul H. Weigel,
Paul T. Englund,
Kenneth Murray,
Robert Old
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.4.1151
Subject(s) - nucleotide , dna , oligonucleotide , endonuclease , base pair , sequence (biology) , bacteriophage , restriction enzyme , terminal (telecommunication) , nucleic acid sequence , symmetry (geometry) , chemistry , microbiology and biotechnology , biology , crystallography , stereochemistry , genetics , escherichia coli , geometry , mathematics , gene , computer science , telecommunications
Analyses of radioactive oligonucleotides in endonuclease digests of 3′-terminally labeled λ DNA revealed the 3′ terminal sequence -GTTACG for thel strand and -ACCCGCG for ther strand. These sequences, together with those previously known for the 5′ cohesive ends, provide a total of 25 known base-pairs in the vicinity of the termini. When the cohesive ends are paired, the sequence between the nicks can be bisected by a 2-fold rotational axis of symmetry. Five of the first eight base-pairs, on either side of the axis, are rotationally symmetric. This symmetry may be involved in the recognition of the site by an enzyme responsible for formation of the cohesive ends.