Open AccessComplex Formation Between Mycobacterial Polysaccharides and Fatty Acyl-CoA Derivatives
Author(s) -
Yuya Machida,
Konrad E. Bloch
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.4.1146
Subject(s) - polysaccharide , chemistry , yield (engineering) , fatty acid , biochemistry , sugar , stereochemistry , metallurgy , materials science
The mycobacterial polysaccharides MMP and MGLP, which contain numerousO -methyl-sugar residues, markedly stimulate fatty acid synthesis catalyzed by a multienzyme complex fromMycobacterium phlei [Ilton, M.et al. (1971)Proc. Nat. Acad. Sci. USA 68, 87-91]. When aqueous solutions containing MMP or MGLP and palmitoyl-CoA were chromatographed on Sephadex G-75 under conditions that widely separate the individual components, polysaccharide and fatty acyl-CoA were eluted in a single peak, indicating formation of a molecular complex. Similarly, the mycobacterial polysaccharides associate with the CoA derivatives of C18 , C20 , and C22 acids to yield complexes containing maximally 1 mol of fatty acyl-CoA per mol of polysaccharide. The formation of these novel complexes may result from hydrophobic interactions between the paraffin chains of the acyl-CoA derivatives andO -methyl-sugar residues of the polysaccharides.