Isolation of Messenger RNA Coding for Mouse Heavy-Chain Immunoglobulin | Zendy

Ronald H. Stevens | Zendy; A. R. Williamson | Zendy

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Isolation of Messenger RNA Coding for Mouse Heavy-Chain Immunoglobulin

Author(s) -

Ronald H. Stevens,

A. R. Williamson

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.4.1127

Subject(s) - rna , messenger rna , microbiology and biotechnology , immunoglobulin light chain , biology , antiserum , immunoglobulin heavy chain , gel electrophoresis , cytoplasm , chemistry , antibody , biochemistry , gene , genetics

A method for purification of the mRNA coding for heavy-chain protein of a mouse G2a myeloma (5563) is reported. Complete myeloma protein (H2 L2 ) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 × 105 and 3.1 × 105 molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated inXenopus laevis oocytes.

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