Open AccessEvidence for the Absence of the G-T-Ψ-C Sequence From Two Mammalian Initiator Transfer RNAs
Author(s) -
Guy Pétrissant
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.4.1046
Subject(s) - rnase p , transfer rna , biochemistry , nucleotide , sequence (biology) , biology , microbiology and biotechnology , chemistry , rna , gene
Analyses were performed on the purified initiator tRNA from rabbit liver to test for the presence of Ψp and Tp in this molecule. Neither of these nucleotides could be detected after hydrolysis by piperidine, NaOH, or T2 RNase. Similarly digestion with venom phosphodiesterase plus phosphatase failed to release any pseudourdine or ribothymidine. Identical results were obtained with the initiator tRNA from sheep mammary gland. The absence of these nucleosides was confirmed by pancreatic and T1 RNase digestion of the rabbit-liver initiator tRNA. The classical G-T-Ψ-C sequence was not detected in this molecule. An A-U-C-G sequence has been identified; it may possibly replace the G-T-Ψ-C sequence.