Open AccessBiosynthesis of Cytokinins in Cytokinin-Autotrophic Tobacco Callus
Author(s) -
John Einset,
Folke Skoog
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.3.658
Subject(s) - cytokinin , zeatin , callus , autotroph , biochemistry , botany , biology , chemistry , auxin , bacteria , genetics , gene
A cytokinin-autotrophic strain of tobacco callus contained cytokinin-active compounds with chromatographic mobilities on Sephadex LH-20 corresponding to ribosylzeatin, zeatin, and 6-Δ2 -isopentenylaminopurine. Zeatin, the apparent major cytokinin, was estimated to be present at a concentration of 10-4 μmol/kg of tissue. Cytokinin-autotrophic callus supplied with [14 C]adenine produced radioactive components with the same chromatographic properties as zeatin and 6-Δ2 -isopentenylaminopurine. These components were not obtained from cytokinin-dependent tissue supplied with [14 C]adenine in the same manner.