Open AccessRegulation of the Expression of the N Gene of Bacteriophage Lambda
Author(s) -
Jack Greenblatt
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.2.421
Subject(s) - bacteriophage , lambda phage , escherichia coli , lysin , biology , in vivo , microbiology and biotechnology , gene , dna , lysogenic cycle , lambda , gene expression , biochemistry , genetics , physics , optics
A quantitative assay for theN protein of bacteriophage λ has been used to study thein vivo regulation ofN gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system fromEscherichia coli programmed with λN - DNA the λ endolysin is made only ifN protein is added to the reaction.The rate of synthesis ofN proteinin vivo is negatively controlled by the products of theCI andtof genes of the phage. Furthermore,N protein activity is extremely unstablein vivo . During normal cell growth at 35°, the half-life ofN protein is about 2 min.