Mitochondrial Protein Synthesis: RNA with the Properties of Eukaryotic Messenger RNA | Zendy

Stanley Perlman | Zendy; H T Abelson | Zendy; Sheldon Penman | Zendy

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Mitochondrial Protein Synthesis: RNA with the Properties of Eukaryotic Messenger RNA

Author(s) -

Stanley Perlman,

H T Abelson,

Sheldon Penman

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.2.350

Subject(s) - five prime cap , messenger rna , rna , mature messenger rna , post transcriptional modification , biology , protein biosynthesis , microbiology and biotechnology , rna dependent rna polymerase , ethidium bromide , biochemistry , mitochondrion , mitochondrial ribosome , rna editing , non coding rna , dna , ribosome , gene

A heterogeneous RNA fraction with properties resembling those of messenger RNA was identified in mammalian mitochondria. Synthesis of contaminating RNA of nuclear origin was suppressed by treatment with camptothecin. Labeling of the messenger-like RNA is completely inhibited by ethidium bromide, a specific inhibitor of mitochondrial functions.Although mitochondrial protein synthesis resembles that of prokaryotes in several regards, the messenger-like RNA is covalently linked to poly(adenylic acid) [poly(A)]. Poly(A) has thus far been found only in eukaryotic cells. The poly(A) segment has a gel electrophoretic mobility of about 4 S, corresponding to a length of 50-80 nucleotides, and thus resembles in size the poly(A) found in some mammalian viral RNAs. The messenger RNA can be released from the mitochondrial protein-synthesizing structure by treatment with puromycin.

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