Open AccessSurface Changes in Temperature-Sensitive Simian Virus 40-Transformed Cells
Author(s) -
Kenneth D. Noonan,
Hartmut C. Renger,
Claudio Basilico,
Max M. Burger
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.2.347
Subject(s) - concanavalin a , wheat germ agglutinin , lectin , biology , microbiology and biotechnology , cell culture , cell , virus , biochemistry , chemistry , virology , in vitro , genetics
A binding assay that shows consistent differences in the amounts of tritium-labeled concanavalin A that bind to normal and virally transformed cells was used to study the kinetic changes of cell surface in SV40-transformed 3T3 cells that express the transformed phenotype in a temperature-sensitive manner (tsSV3T3 cells). The increase in concanavalin A binding, which paralleled the appearance of the characteristics of transformed cells, was dependent on the synthesis of cellular DNA. In agreement with the results of binding studies, exponentially growing tsSV3T3 cells agglutinated at much lower lectin titers (concanavalin A as well as wheat-germ agglutinin) at 32° than at 39°.