Open AccessMutations Affecting the Structure of Hypoxanthine: Guanine Phosphoribosyltransferase in Cultured Chinese Hamster Cells
Author(s) -
Arthur L. Beaudet,
Donald J. Roufa,
C. Thomas Caskey
Publication year - 1973
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.2.320
Subject(s) - hypoxanthine guanine phosphoribosyltransferase , chinese hamster , phosphoribosyltransferase , mutant , adenine phosphoribosyltransferase , hypoxanthine phosphoribosyltransferase , microbiology and biotechnology , enzyme , antiserum , biochemistry , hypoxanthine , biology , hamster , guanine , mutation , gene , chemistry , dna , antibody , genetics , purine , nucleotide
14 Clones resistant to 8-azaguanine, isolated from mutagenically treated cultured cells from Chinese hamsters, were tested for loss of enzymic and immunological activities of hypoxanthine: guanine phosphoribosyltransferase. Three of the clones had no enzymic activity but reacted strongly with antiserum prepared against the enzyme (CRM+ ), indicating the presence of a defective enzyme protein, probably caused by a mutation in its structural gene. The other 11 mutants had little or no enzymic or immunologic activity. Revertant clones with positive enzymic and crossreacting immunologic activities were derived from both classes of mutants. The antiserum has been used for rapid purification of the phosphoribosyltransferase from Chinese hamster cells by immunoadsorbant affinity chromatography.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom