Open AccessImmunological Function of Lymphocytes Fractionated with Antigen-Derivatized Fibers
Author(s) -
Urs Rutishauser,
Peter D’Eustachio,
Gerald M. Edelman
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.12.3894
Subject(s) - antigen , immune system , microbiology and biotechnology , antibody , biology , chemistry , immunology
Specific antigen-binding cells from spleens of immune and nonimmune mice were isolated by the method of fiber fractionation. After removal from the fibers, these cells were assayed for their viability, their ability to rebind to fibers of the same specificity, and theirin vivo response to the antigen after transfer to syngeneic irradiated recipients. These experiments indicate that the fiber method yields highly enriched populations of specific antigen-binding cells that are viable and include antigen-sensitive bone marrow-derived cells capable of undergoing mitosis and differentiating into antibody-secreting cells.