Open AccessInfectious DNA from Herpes Simplex Virus: Infectivity of Double-stranded and Single-stranded Molecules
Author(s) -
P Sheldrick,
Madeleine Laithier,
David Lando,
Marie-Louise Ryhiner
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.12.3621
Subject(s) - endonuclease , infectivity , dna , neurospora crassa , herpes simplex virus , biology , denaturation (fissile materials) , crassa , virus , enzyme , biophysics , virology , microbiology and biotechnology , chemistry , biochemistry , nuclear chemistry , gene , mutant
The infectious units in native and alkalidenatured preparations of DNA of herpes simplex virus were characterized with respect to their sensitivity toNeurospora crassa endonuclease, their sedimentation properties in high-salt, neutral sucrose gradients, and their sensitivity to hydrodynamic shearing forces. Infectious molecules in native preparations were resistant toN. crassa endonuclease, sedimented at 56 S, and were highly sensitive to shearing forces. After alkaline denaturation, infectious molecules became sensitive to theN. crassa enzyme, sedimented at 200 S, and were relatively resistant to shear. We conclude that both intact duplex molecules ([unk]100 × 106 daltons) and intact single strands ([unk]50 × 106 daltons) are capable of initiating productive infection.