English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

I have sequenced the first 63 bases of mRNA transcribed in vitro from the UV5 promoter mutant of the E. coli lactose operon. Sonic fragments of DNA, 1000 base pairs long and purified to contain only the lac operator-promoter region, were used as template. The UV5 promoter mutation allows transcription of the lac operon in the absence of catabolite activator protein and cAMP; lac repressor controls the synthesis of this RNA. I find that during synthesis, RNA polymerase pauses at particular sites along the DNA, naturally generating several discrete sizes of RNA that provide overlaps useful for sequencing. The UV5 lac mRNA initiates within the lac operator and copies the operator sequence. The AUG initiator codon for beta-galactosidase occurs at position 39 of the message. The sequence is: pppA-A-U-U-G-U-G-A-G-C-G-G-A-U-A-A-C-A-A-U-U-U- C-A-C-A-C-A-G-G-A-A-A-C-A-G-C-U-A-U-G-A-C-C-A-U- G-A-U-U-A-C-G-G-A-U-U-C-A-C-U-G-G.

The Nucleotide Sequence of the Lactose Messenger Ribonucleic Acid Transcribed from the UV5 Promoter Mutant of Escherichia coli