Open AccessConformational Control of the Interaction of Eukaryotic Elongation Factors EF-1 and EF-2 with Ribosomes
Author(s) -
César Nombela,
Severo Ochoa
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.12.3556
Subject(s) - ribosome , elongation factor , elongation , ribosomal rna , ef tu , a site , eukaryotic ribosome , protein biosynthesis , chromosomal translocation , translation (biology) , ribosomal protein , chemistry , biology , microbiology and biotechnology , biophysics , biochemistry , binding site , rna , messenger rna , gene , materials science , ultimate tensile strength , metallurgy
As in the case with prokaryotic systems,Artemia salina elongation factors EF-1 and EF-2 interact with a common site or with closely overlapping sites on theArtemia ribosome. This feature of ribosomal design must restrict interaction with the ribosome to only one of the factors at alternating steps of chain elongation. In support of this view we find that EF-1, but not EF-2, interacts with the post-translocation ribosome, whereas the reverse is true of the pre-translocation ribosome. Conformational changes probably account for the alternating selectivity of the translating ribosome for each elongation factor.