Open AccessTissue-Specific Transcription of the Globin Gene in Isolated Chromatin
Author(s) -
Raymond Gilmour,
John Paul
Publication year - 1973
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.12.3440
Subject(s) - biology , transcription (linguistics) , microbiology and biotechnology , chromatin , rna , rna dependent rna polymerase , rna polymerase ii , messenger rna , rna polymerase i , rna polymerase iii , rna polymerase , polymerase , dna , gene , genetics , gene expression , promoter , philosophy , linguistics
RNA was transcribed from chrmation from mouse fetal liver or brain, by use of DNA-dependent RNA polymerase of Escherichia coli. Globin messenger RNA sequences in the transcript were measured with complementary DNA copied from globin messenger RNA with RNA-dependent DNA polymerase. Globin messenger RNA sequences were found in RNA newly transcribed from chromatin from erythropoietic tissue but not in that transcribed from brain chromatin.
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