Open AccessApplication of Fingerprinting Techniques to Iodinated Nucleic Acids
Author(s) -
Hugh D. Robertson,
Elizabeth Dickson,
Peter Model,
Wolf Prensky
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.11.3260
Subject(s) - nucleic acid , rna , oligonucleotide , dna , nucleic acid structure , cytidine , biochemistry , bacteriophage , biology , chemistry , complementary sequences , computational biology , combinatorial chemistry , microbiology and biotechnology , enzyme , gene , escherichia coli , statistics , mathematics
Several techniques of RNA and DNA finger-printing and determination of sequence have been applied to nucleic acids labeled with125 I. Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination method shows little sensitivity to potential structure in single-stranded RNA molecules, yields stable oligonucleotide products in a reproducible manner, and does not change the specificity of several ribonucleases and deoxyribonucleases.