Open AccessStudies on In Vitro DNA Synthesis Isolation of dna B Gene Product from Escherichia coli
Author(s) -
Michel Wright,
Sue Wickner,
Jerard Hurwitz
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.11.3120
Subject(s) - thermolabile , dna , escherichia coli , microbiology and biotechnology , biology , gene , dna polymerase ii , dna clamp , in vitro recombination , mutant , gene product , biochemistry , molecular cloning , complementary dna , polymerase chain reaction , gene expression , enzyme , reverse transcriptase
Formation of duplex DNA from ϕX174 single-stranded DNA by extracts ofE. coli was previously shown to require the gene product ofdna B. Using as an assay the stimulation of ϕX174 DNA-dependent synthesis in inactivated extracts ofdna B temperature-sensitive cells, we purified thedna B gene product from wild-typeE. coli as well as from adna B temperature-sensitive mutant. Thedna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart.