Open AccessImmunochemical Analysis of the Cross-Reacting Idiotypes of Mouse Myeloma Proteins with Anti-Dextran Activity and Normal Anti-Dextran Antibody
Author(s) -
Dennis A. Carson,
Martin Weigert
Publication year - 1973
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.70.1.235
Subject(s) - dextran , myeloma protein , hapten , idiotype , heterologous , antibody , chemistry , immunoglobulin light chain , microbiology and biotechnology , radioimmunoassay , plasmacytoma , multiple myeloma , biochemistry , biology , immunology , monoclonal antibody , gene
The idiotype of the mouse myeloma protein with anti-α-1,3 dextran activity, J558, has been characterized by a solid-phase radioimmunoassay. The idiotype of the J558 protein depends on a specific light and heavy chain interaction and is altered in the presence of the hapten, nigerose. Cross-reacting idiotypes were found on another mouse myeloma protein with α1,3 dextran specificity, normal anti-dextran antibody, and certain reconstructed myeloma proteins composed of the J558 heavy chain and heterologous light chains.