Inhibition of a DNA Polymerase from Bacillus subtilis by Hydroxyphenylazopyrimidines | Zendy

Kenneth B. Gass | Zendy; Robert L. Low | Zendy; Nicholas R. Cozzarelli | Zendy

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Inhibition of a DNA Polymerase from Bacillus subtilis by Hydroxyphenylazopyrimidines

Author(s) -

Kenneth B. Gass,

Robert L. Low,

Nicholas R. Cozzarelli

Publication year - 1973

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.70.1.103

Subject(s) - bacillus subtilis , dna polymerase , polymerase , uracil , dna polymerase ii , dna , microbiology and biotechnology , chemistry , biology , dna polymerase i , dna replication , biochemistry , polymerase chain reaction , genetics , bacteria , gene , reverse transcriptase

The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.

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