Open AccessTransamidase Reactions Involved in the Enzymic Coagulation of Semen: Isolation of γ-Glutamyl-ε-Lysine Dipeptide from Clotted Secretion Protein of Guinea Pig Seminal Vesicle
Author(s) -
H.G. Williams-Ashman,
Angelο C. Notides,
S. S. Pabalan,
L. Lóránd
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.8.2322
Subject(s) - lysine , secretion , dipeptide , guinea pig , seminal vesicle , biochemistry , semen , coagulation , biology , chemistry , amino acid , endocrinology , medicine , anatomy , prostate , genetics , cancer
New supportive evidence is advanced in favor of the hypothesis that the enzymic coagulation of guinea pig semen involves transamidase reactions that result in the formation of γ-glutamyl-ε-lysine intermolecular cross linkages between molecules of a basic protein in seminal vesicle secretion. The dipeptide γ-glutamyl-ε-lysine was isolated in large quantities from proteolytic digests of the coagulated basic vesicular secretion protein that comprises the seminal clot.