Open AccessElectron Microscope Studies of Heteroduplex DNA from a Deletion Mutant of Bacteriophage ϕX-174
Author(s) -
Jung-Suh Kim,
Phillip A. Sharp,
Norman Davidson
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.7.1948
Subject(s) - heteroduplex , trimer , dna , bacteriophage , dimer , biology , mutant , microbiology and biotechnology , monomer , electron microscope , gene , chemistry , genetics , escherichia coli , polymer , physics , organic chemistry , optics
A population of double-stranded replicative form of DNA molecules from bacteriophage ϕX-174 carrying a deletion of about 9% of the wild-type DNA has been discovered in a sample cultivated under conditions where the phage lysozyme gene is nonessential. The structures of deleted monomers, dimers, and trimers were studied by the electron microscope heteroduplex method. The dimers and trimers are head-to-tail repeats of the deleted monomers. Some interesting examples of the dynamical phenomenon of branch migrationin vitro have been observed in heteroduplexes of deleted dimer and trimer strands with undeleted monomer viral strands from the wild-type phage.
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