Open AccessDeletion Mutants of Bacteriophage ϕX174
Author(s) -
Anthony J. Zuccarelli,
Robert M. Benbow,
Robert L. Sinsheimer
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.7.1905
Subject(s) - cistron , mutant , bacteriophage , dna , biology , microbiology and biotechnology , wild type , genetics , gene , escherichia coli
Mutants of bacteriophage ϕX174 have been isolated that are less dense than wild-type ϕX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement ϕX amber mutants in cistronE . We conclude that the mutant viruses have deleted approximately 7% of the ϕX genome in the region of cistronE .