Coupling of a New, Active Morphine Derivative to Sepharose for Affinity Chromatography | Zendy

Eric J. Simon | Zendy; William P. Dole | Zendy; Jacob M. Hiller | Zendy

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Coupling of a New, Active Morphine Derivative to Sepharose for Affinity Chromatography

Author(s) -

Eric J. Simon,

William P. Dole,

Jacob M. Hiller

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.7.1835

Subject(s) - affinity chromatography , derivative (finance) , sepharose , chemistry , coupling (piping) , chromatography , combinatorial chemistry , biochemistry , enzyme , materials science , business , finance , metallurgy

A new, pharmacologically active morphine derivative, 6-succinylmorphine, was synthesized. The properties of this compound and evidence for its structure are presented. Succinylmorphine was covalently coupled to ethylamino-Sepharose. Morphine-Sepharose containing up to 40 mug of morphine did not block the electrically stimulated contraction of isolated guinea pig ileum, but after alkaline hydrolysis of beads containing 2 mug of morphine the supernatant completely blocked contraction. This block was reversed by the specific morphine antagonist naloxone. Antibodies to morphine were removed from serum by morphine-Sepharose, but not by ethylamino-Sepharose, providing evidence of the efficacy of the beads for affinity chromatography.

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