Physicochemical Characterization of Mitochondrial DNA from Pea Leaves
Author(s) -
Richard D. Kolodner,
Κ. Κ. Tewari
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.7.1830
Subject(s) - mitochondrial dna , dna , dna supercoil , intramolecular force , kinetics , circular dna , molecule , chemistry , biology , biophysics , biochemistry , crystallography , stereochemistry , genome , gene , dna replication , physics , organic chemistry , quantum mechanics
The mitochondrial DNA from pea leaves exists in a circular conformation. 25% of the circular molecules exist as supercoils, and 10% of the molecules are dimers. The molecular weight of mitochondrial DNA is about 66 to 70 x 10(6) by electron microscopy, and 74 x 10(6) from its renaturation kinetics. No evidence for inter- and intramolecular heterogeneity is found.
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