Open AccessCharacterization of Yolk DNA from Xenopus laevis Oocytes Ovulated In Vitro
Author(s) -
Françoise Hanocq,
Micheline KirschVolders,
Jacqueline Hanocq-Quertier,
Elyane Baltus,
G. Steinert
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.5.1322
Subject(s) - xenopus , yolk , nuclear dna , dna , biology , mitochondrial dna , microbiology and biotechnology , biochemistry , gene , ecology
High molecular weight DNA was isolated from the yolk platelets ofXenopus laevis oocytes ovulatedin vitro . Yolk DNA has the same buoyant density in CsCl gradients as mitochondrial and nuclear DNAs, and, like them, it is double-stranded. Yolk DNA behaves like nuclear DNA, and not like mitochondrial DNA, upon renaturation after denaturation. The molecules are always linear. Cytochemical and biochemical controls preclude the possibility that the yolk DNA might be contaminated by nuclear or mitochondrial DNA. We conclude that the yolk DNA is an endogenous component of the yolk platelets.