Open AccessThe 3′-Terminal Nucleosides of the High Molecular Weight RNA of Avian Myeloblastosis Virus
Author(s) -
Mary L. Stephenson,
Le Roy S. Wirthlin,
Jesse F. Scott,
Paul C. Zamecnik
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.5.1176
Subject(s) - rna , cytidine , biochemistry , ribosomal rna , transfer rna , microbiology and biotechnology , chemistry , biology , periodate , enzyme , gene
The RNA isolated from avian myeloblastosis virus was fractionated by sucrose density gradient centrifugation. The 3′-OH terminal nucleosides of various fractions were determined by periodate oxidation followed by tritiated borohydride reduction. The 60-70S fraction and the 35S RNA derived from it by heating both have adenosine as the major terminal nucleoside, with cytidine as the next most frequent terminal. Control samples of tRNAmet f. coli and 28S ribosomal RNA from mouse ascites tumor cells gave the expected terminal residues and molecular weights.