Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli | Zendy

Sidney Pestka | Zendy

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Peptidyl-Puromycin Synthesis on Polyribosomes from Escherichia coli

Author(s) -

Sidney Pestka

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.3.624

Subject(s) - puromycin , escherichia coli , polysome , ribosome , protein biosynthesis , chemistry , translation (biology) , biochemistry , biology , rna , messenger rna , gene

Peptide bond synthesis was studied with native polyribosomes ofE. coli . With the use of this system for transpeptidation, it was possible to show that a single K+ activates the ribosome monomers of polyribosomes; that protonation of a single group (probably imidazole or an N-terminal amino group) with a pKa equal to about 7.2 inactivates the transpeptidase complex; that Mn++ can substitute for Mg++ , but that Ca++ , spermidine, and putrescine do so only very poorly; and that theKm for puromycin in this system is about 2.4 × 10-6 M.

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