Inactivation of Ribosomes In Vitro by Colicin E 3 and Its Mechanism of Action | Zendy

Thierry Boon | Zendy

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Inactivation of Ribosomes In Vitro by Colicin E ₃ and Its Mechanism of Action

Author(s) -

Thierry Boon

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.3.549

Subject(s) - colicin , ribosome , ribosomal rna , cleavage (geology) , protein subunit , biology , escherichia coli , in vitro , 50s , bacteriocin , biochemistry , rna , microbiology and biotechnology , bacteria , genetics , paleontology , fracture (geology) , gene

The incubation of purified ribosomes with colicin E(3) results in the cleavage of a terminal fragment from the 16S ribosomal RNA. The cleavage reaction requires three components: colicin E(3), the 30S ribosomal subunit, and the 50S ribosomal subunit. An immunity factor found in extracts derived from colicinogenic cells prevents the in vitro inactivation of ribosomes by colicin E(3). Evidence is presented suggesting that it does so by binding to the colicin molecule. The mode of action of colicin E(3)in vivo can be explained by the assumption that a small fraction of the adsorbed colicin penetrates into the cell and catalytically inactivates the ribosomes.

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