A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells | Zendy

Bertold Fridlender | Zendy; Michael Fry | Zendy; Arthur H. Bolden | Zendy; Arthur Weissbach | Zendy

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A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells

Author(s) -

Bertold Fridlender,

Michael Fry,

Arthur H. Bolden,

Arthur Weissbach

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.2.452

Subject(s) - dna polymerase , polymerase , biology , rna , dna , microbiology and biotechnology , oligonucleotide , rna dependent rna polymerase , dna polymerase ii , biochemistry , gene , reverse transcriptase

Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.

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