Open AccessAction of DNA Polymerase I of Escherichia coli with DNA-RNA Hybrids as Templates
Author(s) -
John D. Karkas,
Jannis G. Stavrianopoulos,
Erwin Chargaff
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.2.398
Subject(s) - escherichia coli , dna , dna polymerase , dna clamp , dna polymerase ii , dna polymerase i , polymerase , biology , microbiology and biotechnology , rna polymerase , chemistry , genetics , rna , computational biology , gene , reverse transcriptase
Experiments indicating the ability of the ribo strand of a DNA-RNA template to guide polydeoxynucleotide synthesis by highly purified DNA polymerase I of E. coli (EC 2.7.7.7) are presented. With poly(rA).poly(dT) as template, poly(dT) is formed with a high efficiency, but almost no poly(dA). The specific activity of the enzyme, when tested with this template under suitable conditions, is eight times greater than that found for the poly(dA-dT) template. Single-stranded DNA fractions, with no template activity for DNA polymerase, are converted to efficient templates after their transcription by RNA polymerase. A concerted polymerization reaction, in which the action of DNA polymerase is dependent on that of RNA polymerase, can also be demonstrated with synthetic polydeoxynucleotides and single-stranded fractions of denatured DNA as templates.
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