A Ribosome Dissociation Factor from Rabbit Reticulocytes | Zendy

Nicolette H. Lubsen | Zendy; Bernard D. Davis | Zendy

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A Ribosome Dissociation Factor from Rabbit Reticulocytes

Author(s) -

Nicolette H. Lubsen,

Bernard D. Davis

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.2.353

Subject(s) - ribosome , reticulocyte , escherichia coli , ribosomal rna , dissociation (chemistry) , transfer rna , chemistry , biochemistry , initiation factor , protein biosynthesis , biology , a site , messenger rna , biophysics , microbiology and biotechnology , rna , binding site , gene

A ribosome dissociation factor has been detected in an extract of ribosomal particles from rabbit reticulocytes. This factor dissociates free ribosomes from reticulocytes and also from Escherichia coli; it does not dissociate ribosomes complexed with peptidyl-tRNA and mRNA. The reaction appears to be stoichiometric rather than catalytic; it reaches completion in one minute at 37 degrees C, but is very slow at 0 degrees C, and it is antagonized and reversed by Mg(++). Reticulocyte dissociation factor thus closely resembles that from E. coli. However, the activity has been found primarily associated with the native large subunits rather than the small subunits in lysates.

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