The Sensitivity of Developing Cardiac Myofibrils to Cytochalasin-B | Zendy

Francis J. Manasek | Zendy; Beth Burnside | Zendy; John Stroman | Zendy

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The Sensitivity of Developing Cardiac Myofibrils to Cytochalasin-B

Author(s) -

Francis J. Manasek,

Beth Burnside,

John Stroman

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.2.308

Subject(s) - myofibril , cytochalasin b , cytochalasin , myofilament , myosin , biology , microbiology and biotechnology , anatomy , cytochalasin d , biophysics , embryo , actin , sarcomere , myocyte , cytoskeleton , biochemistry , cell

Developing cardiac muscle cells of 11- to 13-somite chick embryos are sensitive to cytochalasin-B. In cultured chick embryos, ranging in development from 11 to 13 somites, hearts stop beating in the presence of this agent. Both polarized light and electron microscopic examination show that cytochalasin-B disrupts existing myofibrils and inhibits the formation of new ones. Discrete Z-bands are not present in treated heart cells and thick, presumably myosin, filaments are found in disarray. These effects are reversible; after cytochalasin-B is removed from the medium, heartbeat recovers and myofibrils with discrete Z-bands reappear. Fibrillar sensitivity appears to be a function of age since fibrils in hearts of embryos having from 22 to 28 pairs of somites are more resistant.

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