Open AccessAltered Chloroplast Ribosomal Proteins Associated with Erythromycin-Resistant Mutants in Two Genetic Systems of Chlamydomonas reinhardi
Author(s) -
Laurens Mets,
Lawrence Bogorad
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.12.3779
Subject(s) - chlamydomonas , ribosomal protein , biology , mutant , genetics , ribosome , chloroplast , mutation , protein subunit , ribosomal rna , gene , rna
The phenotype of several erythromycin-resistant mutants ofChlamydomonas reinhardi was further characterized in terms of the electrophoretic properties of their chloroplast ribosomal proteins. In mutantery-M2d a single protein of the large (52 S) subunit has altered properties, which probably result from a change in its primary sequence. This mutation is inherited in a Meudelian manner. In mutantery-U1a , which is inherited in a uniparental manner, a different single protein of the 52 S subunit is altered. This change might result from a change in either the primary sequence of the protein or in some form of secondary modification. These results indicate that these two distinct genetic systems must cooperate in the production of chloroplast ribosomes.