Release of Polypeptide Chain Initiation Factor IF-2 During Initiation Complex Formation | Zendy

Arthur H. Lockwood | Zendy; Probir Kumar Sarkar | Zendy; Umadas Maitra | Zendy

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Release of Polypeptide Chain Initiation Factor IF-2 During Initiation Complex Formation

Author(s) -

Arthur H. Lockwood,

Probir Kumar Sarkar,

Umadas Maitra

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.12.3602

Subject(s) - gtp' , initiation factor , ribosome , eukaryotic initiation factor , chemistry , eif2 , eukaryotic translation , polypeptide chain , complex formation , biochemistry , translation (biology) , biophysics , microbiology and biotechnology , stereochemistry , biology , rna , messenger rna , enzyme , gene , inorganic chemistry

Polypeptide chain initiation factor IF-2 binds to 30S ribosomal subunits. This binding is enhanced by IF-1 and IF-3. During GTP-dependent formation of a 70S initiation complex, IF-2 is released from the ribosome. During 70S initiation complex formation dependent on the methylene analogue of GTP, GMPPCH2 P, IF-2 is not released, but remains bound to the 70S ribosome. This result suggests that IF-2 release requires GTP hydrolysis. In agreement with this presumed requirement, IF-2 functions catalytically with GTP, but stoichiometrically with GMPPCH2 P, in bringing about 70S initiation complex formation.

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