Open AccessA Mutant of Escherichia coli Defective in DNA Polymerase II Activity
Author(s) -
Yukinori Hirota,
Malcolm L. Gefter,
Leonard Mindich
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.11.3238
Subject(s) - mutant , escherichia coli , mutation , biology , polymerase , dna polymerase , microbiology and biotechnology , dna , dna polymerase i , mutagenesis , genetics , polymerase chain reaction , gene , reverse transcriptase
A mutant ofE. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to replicate DNA of various sources was analyzed. Mutant bacteria grow normally, at 41° as well as at 30°. All bacteriophages, F factors, and R factors examined so far grow normally in the mutant. Sensitivity of the mutant to ultraviolet radiation and alkylating reagents in growth media was the same as that of the wild type.The mutation,polB1 , is recessive with respect to wild type. ThepolB1 mutation can coexist with recombination-defective mutations. Genetic mapping studies show the mutation to be located at about 2 min on theE. coli map.