Open AccessA Transcribing Activity Induced by Satellite Phage P4
Author(s) -
Kathleen J. Barrett,
Warren Gibbs,
Richard Calendar
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.10.2986
Subject(s) - bacteriophage , enzyme , polymerase , satellite , mutant , dna , phagemid , biology , microbiology and biotechnology , dna polymerase , rna , rna polymerase , chemistry , biochemistry , escherichia coli , gene , physics , astronomy
Satellite bacteriophage P4 induces a new transcribing enzyme that synthesizes polyriboguanylic acid in the presence of the poly(dG)·poly(dC) homopolymer pair. This transcribing activity was partially purified and shown to be distinct from the host RNA polymerase. Analysis of conditional lethal mutants suggests that this new enzyme is necessary for replication of phage DNA.