Open AccessActivation of Membrane-Bound Adenylate Cyclase by Glucagon in Neurospora crassa
Author(s) -
Mirtha M. Flawiá,
Héctor N. Torres
Publication year - 1972
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.10.2870
Subject(s) - glucagon , glycogen phosphorylase , neurospora crassa , adenylate kinase , glycogenolysis , cyclase , glycogen , neurospora , biology , biochemistry , incubation , medicine , enzyme , endocrinology , chemistry , hormone , mutant , gene
Membrane-bound adenylate cyclase in Neurospora crassa is activated by glucagon. Half-maximal effect is observed at hormone concentrations of about 10 nM. After solubilization of the enzyme with Lubrol-PX, the glucagon effect is lost. Incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (EC 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (EC 2.4.1.1) and in the rate of glycogenolysis.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom