Open AccessElectron Microscopic Studies of Replicating and Catenated Colicin Factor E1 DNA Isolated from Minicells
Author(s) -
Motohiro Fuke,
Joseph Inselburg
Publication year - 1972
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.69.1.89
Subject(s) - dna , molecule , colicin , chemistry , crystallography , stereochemistry , biophysics , biology , biochemistry , plasmid , organic chemistry
Replicating and catenated DNA molecules of colicin factor E1 that were isolated from minicells were observed by electron microscopy. Two new replicating structures that have been found include: molecules that contain two untwisted replicated branches of the same length and a twisted unreplicated branch, and a twisted circular molecule with a long tail. Other molecular forms identified include circular dimers, three types of catenated dimers (open-open, open-twisted, and twisted-twisted), catenated dimers in which one of the linked molecules is replicating, catenated trimers, and a tetamer.