Geometry of the First Step in the Action of Ribonuclease-A | Zendy

D. A. Usher | Zendy; Evelyn S. Erenrich | Zendy; F. Eckstein | Zendy

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Geometry of the First Step in the Action of Ribonuclease-A

Author(s) -

D. A. Usher,

Evelyn S. Erenrich,

F. Eckstein

Publication year - 1972

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.69.1.115

Subject(s) - cytidine , ribonuclease , uridine , action (physics) , line (geometry) , chemistry , stereochemistry , s tag , geometry , physics , rna , mathematics , biochemistry , enzyme , quantum mechanics , gene

Advantage was taken of the reversibility of the first step of ribonuclease-A action to synthesize the dinucleoside phosphorothioate Up(S)C from the crystalline isomer of uridine 2',3'-cyclic phosphorothioate [Up(S)] and cytidine. Cyclic phosphorothioate was then reformed from Up(S)C by a nonenzymic reaction known to proceed by an in-line mechanism. The geometry of the enzymic reaction was determined to be in-line by a comparison of the Up(S) product with the Up(S) originally used. By the principle of microscopic reversibility, the geometry of the first step in the action of ribonuclease-A is shown to be in-line.

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