Open AccessPurification of the araC Protein
Author(s) -
Gary Wilcox,
Kenneth J. Clemetson,
Daniel V. Santi,
Ellis Englesberg
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.9.2145
Subject(s) - operon , l arabinose operon , arabinose , inducer , sepharose , affinity chromatography , biochemistry , chemistry , lac operon , gene , covalent bond , gene product , regulator gene , microbiology and biotechnology , biology , regulation of gene expression , gene expression , enzyme , escherichia coli , organic chemistry , xylose , fermentation
The araC gene product, a regulatory protein required for expression of the L-arabinose operon, has been purified by affinity chromatography on Sepharose 4B to which 4-aminophenyl-beta-D-6-deoxygalactopyranoside (an anti-inducer of the L-arabinose operon) had been covalently attached by means of a 4-aminophenylbutanamido side chain. Evidence is presented showing that the protein binds specifically to ara DNA.