Open AccessControl of Plasmid Replication in Escherichia coli: Correlation of the Membrane Site of DNA Replication with the Bacterial Segregation Unit
Author(s) -
David Korn,
Marjorie Thomas
Publication year - 1971
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.9.2047
Subject(s) - biology , replicon , lysogenic cycle , dna replication , prophage , bacterial conjugation , dna , origin of replication , bacterial genome size , genetics , circular bacterial chromosome , genome , semiconservative replication , plasmid , escherichia coli , microbiology and biotechnology , bacteriophage , gene
The fate of parental lambda genomes after superinfection of homoimmune lysogenic cells was studied. The data confirm a previous observation that in the presence of the lambda repressor, superinfecting lambda DNA does not become associated with replication sites on the bacterial cell membrane. Under these conditions, the nonreplicating, superinfecting phage genomes do not become associated with the bacterial segregation unit. These results support the concept that the attachment of DNA to the bacterial membrane at specific sites is involved in the control of both chromosome replication and segregation, as predicted by the replicon hypothesis.
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